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KMID : 0861020170320010001
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Korea Journal of Herbology
2017 Volume.32 No. 1 p.1 ~ p.13
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Enhancement of Immune Activities of Canavalia gladiata & Arctium lappa complexes in immobilization stress mouse model.
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Lee Ji-Eun
Roh Seong-Soo
Kim Han-Young
Kim Kun-Hoae
Kim Seung-Hyung
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Abstract
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Objective : Soybeans of Canavalia gladiata (CG) and root of Arctium lappa (AL) have been reported to have antiinflammatory, antioxidant effect. However, the immunoregulatory mechanisms of its combinational prescription remain a matter of considerable debate. In the current study, we investigated whether CG and AL and its combinational prescription(CG+AL) regulate immune system using chronic immobilization-stress mouse model.
Methods : C57BL/6J mice fixed for 2 hours into immobilization tube after CG, AL, CG+AL oral administration after 2 hours daily for 21 days. After every experiment has ended the C57BL/6J mice were sacrificed on 22 days. The production of Serotonin and Cortisol, lgA were observed by ELISA method, The proportion of immune cells such as T/B cell and macrophage, NK cell were measured by FACS. Then, Real-time PCR was performed to measure the mRNA expression of Inflammatory cytokines(IL-1beta, IL-6, TNF-a) and T cell activation cytokines(IL-2, IL-10, IFN-gamma, IL-12p35 / p40).
Result : When chronic immobilization-stress mouse model were treated with CG+AL(1:4), the expression of mRNA were significantly decreased at the Inflammatory cytokines(IL-1beta, IL-6, TNF-a). While, the levels of mRNA were significantly increased at immune T cell activation cytokines. Additionally, CG+AL(1:4) combinational prescription group enhanced immune cells such as T/B cell and macrophage, NK cell. Furthermore, the Immuno-fluorescence result of brain tissue can confirm that CG+AL(1:4) group significantly increased the BDNF expression.
Conclusion : These result suggest that CG+AL(1:4) combinational prescription has Immune System enhancement via stress-mediated immunocyte.
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KEYWORD
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Enhancement of Immune Activities, soybeans of Canavalia gladiata DC semen, root of Arctium lappa, chronic immobilization-stress, Cytokine
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